Surfactants are ubiquitous interfacial materials that govern the behavior of countless biological, chemical, and industrial systems. Among them, pulmonary surfactant represents a particularly important biological example, forming a complex phospholipid–protein film that maintains alveolar stability by lowering surface tension at the lung air–liquid interface. Understanding the biophysical behavior of such surfactant systems requires specialized instrumentation capable of probing interfacial properties with high sensitivity, temporal resolution, and physiological relevance. Consequently, the development of advanced surfactometry techniques has become a critical area of research. These techniques provide indispensable tools for quantifying surface tension, adsorption kinetics, film compressibility, phase behavior, and dynamic interfacial responses, thereby enabling mechanistic studies of surfactant function, dysfunction, and therapeutic intervention.